足球比分网

樣本保存方法及冷凍脅迫對蝗蟲基因組DNA提取的

時間:2019-09-06 09:53來源:未知 作者:ydgmve2020yyx 點擊:
以亞洲小車蝗為研究材料,提出了使用液氮保存蝗蟲樣本的有效方法,并用基因組DNA提取試劑盒分別對液氮速凍后保存、直接冷凍、無水乙醇保存和干制蝗蟲標本進行了基因組DNA的提取

以亞洲小車蝗為研究材料,提出了使用液氮保存蝗蟲樣本的有效方法,并用基因組DNA提取試劑盒分別對液氮速凍后保存、直接冷凍、無水乙醇保存和干制蝗蟲標本進行了基因組DNA的提取和電泳檢測。MVE液氮罐

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1.3.2ZHIJIELENGDONGBAOCUN

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1.4DNADETIQU

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足球比分网 1.5DNADIANYONGJIANCE

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2.1YEDANSUDONGCHULIDUIYANGBENBAOCUNDEYINGXIANG

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足球比分网 2.2BUTONGBAOCUNFANGFADUIJIYINZUDNATIQUYINGXIANG

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足球比分网 2.3LENGDONGXIEPODUIJIYINZUDNATIQUYINGXIANG

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實驗結果:在保存3個月后,檢測蝗蟲樣本直接冷凍法和干標本提取的總DNA濃度較低,因而瓊脂糖電泳檢測亮度低;液氮速凍后保存和無水乙醇保存的蝗蟲樣本提取的基因組DNA濃度大。表明液氮速凍后保存和無水乙醇保存的標本適合用于基因組學研究。通過對比,直接冷凍保存與液氮速凍保存結果得出,蝗蟲在冷凍脅迫死亡的過程中有DNA降解發生。MVE液氮罐

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